This Mouse Tumor Necrosis Factor Receptor Superfamily Member 22 ELISA Kit from Innovative Research is intended for quantitative detection of mouse TNFRSF22 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.
This mouse TNFRSF22 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF22 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E42-R198) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF22 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF22 amount of sample captured in plate.
- Detection Target: Tumor Necrosis Factor Receptor Superfamily Member 22
- Uniprot ID: )
- Reactivity: Mouse
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 15.6pg/ml-1000pg/ml
- Sensitivity: <10pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: TNFRSF22 interacts via a complex receptor system to preferentially induce apoptosis of tumor cells in vivo and in vitro. This gene is mapped to 7 F5; 7 88.27 cM. The mouse decoy TRAIL R2(TNFRSF22) gene encodes a 198 amino acid (aa) residues type I transmembrane protein with a putative 39 aa signal peptide, a 136 aa extracellular domain, a 18 aa transmembrane region, and a 5 aa cytoplasmic tail. An alternatively spliced variant encoding a secreted soluble receptor of 180 aa has also been described. The transmembrane and soluble receptors are known as mDcTRAIL R2L and mDcTRAIL R2S, respectively. Both mDcTRAIL R2 isoforms and the GPI-linked mDcTRAIL R1 bind TRAIL specifically but are incapable of triggering signal transduction. Mouse DcTRAIL R1 and mDcTRAIL R2 share 71% sequence identity. The two receptors have similar structural modules in their CRDs, which differ from those found in the CRDs of mTNF RH3. Mouse DcTRAIL R1 preferentially binds mTRAIL while mDcTRAIL R2 binds both human and mTRAIL. mRNA of mouse DcTRAIL R1 and -2 are expressed at low levels in many mouse tissues and at higher levels in several mouse cell lines.; Immunogen sequence: 369
